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Real-time qPCR was performed using GoTaq qPCR Master Mix (Promega, A6002) in a QuantStudio 6 Flex thermocycler (Applied Biosystem) or 7900HT Fast Real-Time PCR System (Thermo Fisher)
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Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase
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However, retatrutide has not received approval from the MHRA or the EMA and remains an investigational agent undergoing Phase 3 trials
Note: This product alone is not intended to diagnose, treat, cure, or prevent any disease
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