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Antioxidants are molecules that reduce oxidative stress at the cellular level

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We conducted molecular docking simulations of various TMQ derivatives targeting the GDP-binding site of OPA1

This stress occurs when unstable molecules called free radicalswhich come from things like pollution, UV rays, and normal metabolic processesdamage your cellular structures

The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)