PMID: 22537212 | DOI: 10.1111/j.1753-4887.2012.00476.x [21] Machowetz A, Poulsen HE, Gruendel S, et al
Human studies have used differing forms, doses and treatment periods
the first step is conjugation of glutamic acid and cysteine, which is catalyzed by glutamate-cysteine ligase and generates -glutamyl-cysteine (-Glu-Cys) and the second step is catalyzed by glutathione synthase, which adds glycine to -Glu-Cys, forming glutathione
Crystallization of water during freezing and subsequent thawing can mechanically damage the peptide structure
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