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GR activity (U/L) = (dOD s / t) x (440 / (OD-CAL - OD-buffer)) x n, where OD-CAL and OD-buffer are the OD 412nm values of the Calibrator and Assay Buffer, t is the reaction time (20 min recommended) and n is the dilution factor

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The decision comes down to the mechanism under study: KPV for isolated inflammatory pathway research, BPC-157 for gut barrier structure, angiogenesis, or broader tissue-repair research questions.