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Targeted regulation of the Ras1-cAMP-PKA signaling pathway has inhibitory effects on the transformation of yeast into hypha and the formation of biofilms in vitro , and reduces the fungal load in a mouse systemic fungal infection model (Wang et al., 2024)

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To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig

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