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We therefore quantified neural activity by comparing fluorescent levels to the first frame Extraction of the pulsatile activity in smooth gradients Pulses were extracted automatically using the following criterion: a local maximum with an amplitude that exceeds 20% of the maximal measured fluorescence, and which is surrounded by a decay of at least 70% of its amplitude

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Notably, although the extent of weight loss is reduced in both CNS GLP-1R KO and CNS GIPR KO mice treated with mGIPR-Ab/GLP-1, it is not completely abolished, suggesting that each receptor contributes to the weight loss observed in control mGIPR-Ab/GLP-1-treated mice