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Bacteriostatic water contains 0.9% benzyl alcohol as a preservative

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For drug treatments and qPCR analyses, hiPSC-CM were seeded on gelatin- or Matrigel-coated 12-well plates at 500,000 cells/well in medium containing RPMI 1640, B-27 supplement, 10 M ROCK inhibitor and 10% fetal bovine serum