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Taking a typical enzymatic synthesis reaction as an example, first, amino acids protected by amino groups and amino acid substrates protected by carboxyl groups are mixed in the presence of deprotective agents, and then enzymes are added to catalyze the formation of peptide bonds

TABLE 10 Structures of six metabolites identified by high-performance liquid chromatography-tandem mass spectrometry in rat plasma, bile, urine, and feces following a single intramuscular administration of 100 g/300 Ci/kg of [3H]BPC157

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