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After blocking with 5% BSA in PBS for 1 h, the PVDF membranes were incubated with anti-GFP antibody (Mouse-polyclonal anti-GFP Sigma Aldrich 11814460001 - 1:1000) and -tubulin (Mouse monoclonal anti-alpha-tubulin (clone 12G10) Developmental Studies Hybridoma Bank AB1157911 - 1:200) in 1% BSA in PBS-tween (0.1%) for 16 h at 4 C
Kastin AJ, Akerstrom V
Could B12 deficiency cause weight changes
Hydroxy acid: An carboxylic acid containing an additional hydroxy group (-OH), substistuted to another carbon atom than the acid group.
Understanding these risks is crucial for making an informed decision about starting treatment
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