Our group also recently demonstrated that cancer cells increase SHMT2-driven dependency on the ser/gly synthesis pathway upon radiotherapy treatment
Quantitative realtime PCR (qPCR) was carried out on an Agilent Mx3000P system using the cycling conditions of 95 C for 5 min for initial denaturation, followed by 40 cycles of 95 C for 10 s and 60 C for 30 s
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