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Protein extracts were then subjected to SDSPAGE and transferred to Immobilin-FL polyvinylidene fluoride transfer membrane

For a 5 mg vial, adding 1-2 mL works because you will likely use the entire vial in one or two doses

While the animal model treated with PBS did not show any signs for ROS, high amounts of fluorescence signals in the tissue from the animal treated with NP4 were observed (Supplementary Fig

Wiley-Blackwell, Chichester Anselmo AC, Mitragotri S (2016) Nanoparticles in the clinic

Kasthuri N, Hayworth KJ, Berger DR, Schalek RL, Conchello JA, Knowles-Barley S, et al