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Proliferation (log 2 doublings, 5 days) of indicated GPX4-deficient cell lines in vitro left untreated or supplemented with -toc, vitamin D3, or 7-DHC at indicated concentrations

doi: 10.1136/ard-2022-222764 20 MaiuoloJOppedisanoFGratteriSMuscoliCMollaceV

Clinically overt vitamin B12 deficiency, characterized by hematological and neurological dysfunctions, is relatively uncommon

The trade-off is pharmacokinetic: oral delivery subjects the peptide to stomach acid (pH roughly 1.5-2.0 fasting) and proteolytic enzymes including pepsin and pancreatic proteases before any absorption can occur