One distinctive feature of these compounds is a 4.5% cobalt content, hence the name cobalamin" (cobalt + vitamin)
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Formula: Concentration (mg/ml) = Powder amount (mg) Water volume (ml) Common scenarios: 50mg vial + 2.5ml water = 20mg/ml For 1mg dose: 1 20 = 0.05ml = 5 units on U-100 syringe For 2mg dose: 2 20 = 0.1ml = 10 units 50mg vial + 2ml water = 25mg/ml For 1mg dose: 1 25 = 0.04ml = 4 units For 2mg dose: 2 25 = 0.08ml = 8 units 100mg vial + 5ml water = 20mg/ml For 2mg dose: 2 20 = 0.1ml = 10 units For 3mg dose: 3 20 = 0.15ml = 15 units Use the peptide reconstitution calculator to verify your math before mixing
Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter
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This is key to quickly fix the deficiency and ease symptoms