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Matrix degradation as collagen, elastin, and hyaluronic acid production declines while breakdown increases

Dual luciferase reporter assay For the luciferase assay, 293 T cells were cultured in 24-well plates at 70 to 80% confluency and co-transfected with the plasmids encoding Ppara -wild, Ppara -mut, Pparg -wild, or Ppard- wild, along with the PPRE plasmid and a control vector (Renilla luciferase), using Lipofectamine 3000 Reagent (Invitrogen, L3000150) according to the manufacturers protocols

Natural sources The highest concentrations of carnitine are found in red meat and dairy products

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Fat-burning supplements including L-carnitine-based ones do not work like a magic bullet

This is one of the questions patients most frequently arrive with because theyve often been dismissed by their GP or gastroenterologist before seeking a functional medicine evaluation