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NAD+ vs NADH: The Redox Pair That Powers Cellular Metabolism Understanding why NAD Plus is the same as NAD+ requires understanding why NAD+ and NADH are not the sameand why the oxidation state matters for biological function
N.SriramR.Villanueva-MeyerJ
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Briefly, tissues were homogenized in 500 L of RNAiso plus, with total RNA separated by mixing with 100 L of chloroform, and isolated by isopropanol precipitation
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