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Sleep is equally relevant: testosterone is primarily produced during sleep, and research has found that even one week of sleeping five hours per night reduces testosterone levels by approximately 10 to 15% in young men, a reduction equivalent to ageing ten to fifteen years

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This approach can help simplify research protocols, improve consistency, and reduce preparation time while maintaining accurate peptide ratios throughout a study

Front Endocrinol 14:1133000 Huang J, Xie L, Song A, Zhang C (2022) Selenium status and its antioxidant role in metabolic diseases