Trono (Ecole Polytechnique Federale de Lausanne, Lausanne, Swisse)] and MISSION pLKO.1-puro-based vectors (Sigma-Aldrich, Madrid, Spain,) encoding either a non-targeting shRNA (SHC002) or GCLC -targeting shRNAs sh-62 (TRCN0000344862), sh-65 (TRCN0000333565), and sh-86 (TRCN0000048486)
The IgG binding ability was utilized to produce an affinity tag-free system for direct purification of IgGs from serum, cell supernatants as well as extracts
Thats not going to happen at Costco, Apovian said
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