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Moreover, islets extracted from high cholesterol-fed mice showed decreased exendin-4-induced cAMP responses ex vivo (Supplementary Figure 1A), suggesting a direct impact of increased cholesterol levels on GLP-1R signalling

Hybridization solution (100 l) was dispensed into the gasket slide and assembled to the gene expression microarray slide, and the slides were incubated for 17 h at 65C

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