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Experimental Reagents and materials Bovine serum albumin (96%, BSA, Sigma) was purchased in lyophilized-powder form and used without further purification

it is a regulatory one that limits peak tissue receptor activation

After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Literature both supporting and opposing them are available

doi: 10.1111/ijcp.14618

The methylation of DNA is particularly important, as it helps to regulate gene expression and maintain the integrity of the genome