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Briefly, 100 l of enzyme conjugate was added to the wells containing 50 l of standard or plasma, covered with an adhesive strip and incubated for 60 min at 37 C
Plasma metabolites are not cardiac-specific
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Results LPL treated with deoxycholate prevents formation of inactive helices To generate a homogenous sample of active LPL for cryoEM, we needed to disrupt the inactive LPL helices that form at high protein concentrations
Eur J Biochem 1980
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