In cultured human keratinocytes exposed to UVB radiation (a standard oxidative stress model), pre-treatment with 10 nM GHK-Cu resulted in: 2.8-fold increase in glutathione reductase mRNA (the enzyme regenerating reduced glutathione from its oxidized form) 2.3-fold increase in catalase expression (HO decomposition) 1.9-fold increase in ferritin heavy chain (sequestering iron, another Fenton-active metal) 62% reduction in 8-hydroxy-2-deoxyguanosine (8-OHdG) levels, a DNA oxidative damage marker The protective effect disappeared when using copper-free GHK or copper chloride alone, confirming the intact peptide-copper complex is required
In terms of compound-specific publications, BPC-157 has a larger body of peer-reviewed literature, much of it from the Sikiri group at the University of Zagreb
Adding the 9mg arm suggests the sponsor believes the 8mg to 12mg range contains the optimal therapeutic dose and wants more granular data to identify it precisely
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