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FIGURE 8 3.8 Immunohistochemical findings The immunohistochemical investigation demonstrated a significant increase in expression of proapoptotic makers, Bax, and caspase-3, in MTX treated group, which was associated with decreased antiapoptotic marker, Bcl-2, expression when compared to the CTRL group (Figure 9)

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Mechanistically, appetite suppression and delayed gastric emptying can undermine caloric intake and glycogen repletion, posing risks for endurance, power output, and recovery in elite athletes

5.3 Metabolites The metabolite cargo of small EVs, mainly lipids, varies depending on the origin and metabolic state of the cells