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For histology, mouse livers were fixed in 4% paraformaldehyde in PBS at 4C for 24 h and then switched to 70% ethanol

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The cells were then treated in triplicate with 2012, 2020 (negative control), and Gambogic Acid (positive control) at titrating concentrations ranging from 100 M to 200 nM and allowed to incubate for 45 min in a 37 C incubator with 5% CO 2

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Primarily for reconstituting lyophilized peptides and other research compounds requiring multiple sterile draws over time