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The qRT-PCR primer sets for cDNA amplification were as follows: TNF- (forward: 5-TCCAGGCGGTGCCTATGT-3/reverse: 5-CACCCCGAAGTTCAGTAGACAGA-3), IL-6 (forward: 5-CTGCAAGAGACTTCCATCCAGTT-3/reverse: 5-AAGTAGGG AAGGCCGTGGTT-3), IFN- (forward: 5-TCAAGTGGCATAGATGTGGAAGAA-3/reverse: 5-TGGCTCTGCAGGATTTTCATG-3), CXCL2 (forward: 5-CTCAGTGC TGCACTGGTCCTG-3/reverse: 5-CTGGGGGCGTCACACTCAAG C-3), and GAPDH (forward: 5-CCTCGTCCCGTAGACAAAATG-3/reverse: 5-TCTCCACTTT GCCACTGCAA-3)

Genes involved in tissue repair, collagen synthesis, and antioxidant protection see increased activity, while genes associated with inflammation, tissue breakdown, and cellular aging see reduced expression

We keep a close eye on how patients react to treatment and adjust the dose as needed

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FOXO4-DRI enters senescent cells and competitively binds to p53 through its modified peptide sequence, displacing endogenous FOXO4 and freeing p53 to activate cell death pathways including BAX and PUMA

JASPAR 2016: a major expansion and update of the open-access database of transcription factor binding profiles