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Whole-mount embryo alkaline phosphatase staining and ISH For alkaline phosphatase staining, E8.5 embryos were fixed in 4% paraformaldehyde for 15 min at room temperature or overnight at 4 C and then washed twice with TBST
Livingstone SJ, Levin D, Looker HC, Lindsay RS, Wild SH, Joss N, et al
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