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Nevertheless, under physiological conditions, in most tissues mitochondria are the major contributors to the cellular ROS production [28,29,30,31], mainly as a side effect of aerobic respiration via oxidative phosphorylation (OXPHOS) occurring on the inner membrane of the mitochondrion [32, 33], but also through other metabolic routes, such as pentose-phosphate pathway and glutathione (GSH) metabolism [34]

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Dundar HA, Kiray M, Kir M, Kolatan E, Bagriyanik A, Altun Z, Aktas S, Ellidokuz H, Yilmaz O, Mutafoglu K, Olgun N (2016) Protective effect of acetyl-L-carnitine against doxorubicin-induced cardiotoxicity in Wistar albino rats

While these shots are generally considered safe for long-term use, their effectiveness is most prominent when combined with healthy lifestyle changes

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