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Rather than focusing only on NAD + in isolation, its important to understand where it fits within the complex system and how it can be optimized in the context of improving performance of the whole system

Quantification of cerebral infarct size In brain matrix (RWD Lifesciences), 2 mm coronal sections of the brain were cut out, and they were incubated for 30 min at 37 C in 1% 2,3,5-Tetrazolium chloride (TTC) solution in 0.1 M PBS

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