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Similarly, resistance to oxidative attack increased after overexpression of GPX5 in CHO-K1 cells, and GPX5 overexpressing cells reduced lipid peroxidation levels and downstream DNA damage marker 8-OXODG levels compared to control cells (Taylor et al., 2013)

After thawing, the pellets were lysed in MTTB buffer (50 mM Tris, 150 mM NaCl, 1% (w/v) Triton X-100, 0.2 mM phenylmethylsulfonyl fluoride (PMSF), aprotinin (5 mg/ml), leupeptin (10 mg/ml) and pepstatin A (1 mg/ml))

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Ensure that the needle is bevel up and that you fully insert it to reach the muscle

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