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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat

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The compound has not progressed through a drug-approval pathway in any major Western jurisdiction, although Russian-federation peptide-bioregulator research has produced extensive preclinical and clinical-stage work over more than two decades

86, 87 In patients for whom Hunner lesions are suspected, it is appropriate to proceed to cystoscopy in order to assess for the presence of Hunner lesions (Statement 4)

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