Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems
A number of studies have found that GLP-1 receptor agonists can 9: Reduce body weight and waist circumference Improve insulin resistance (measured by HOMA-IR) Lower fasting glucose and insulin levels These changes may contribute to improvements in ovulatory function and menstrual regularity, particularly in individuals with overweight or obesity
26 GLP-1 and effects on bone metabolism Several studies show that the GLP-1R is expressed in some bone cells, including osteoclasts and BMSCs, but appears to be absent in mature osteoblasts, indicating a complex role in bone homeostasis that is incompletely understood
10.1038/nature22800 36 JoS.KimT.IyerV
Pullar, Anitra C
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