However, this GSH-N-ethylmaleimide derivatization logistically was not possible for us as it is required during the sample collection, and we used sedimented erythrocytes provided to us from another study
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Add 100 L prepared HRP-Secondary antibody solution
we first recapitulated these findings and confirmed that omnivores ( n = 15) have a much greater capacity to generate d 3 -TMAO from orally ingested d 3 -l-carnitine than vegans and vegetarians ( n = 9) (Figure 3A, left panel)