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The randomization sequence was kept in a sealed, opaque envelope, which was opened sequentially by the recruiting team upon patient enrollment

The medium was removed and 125 I-GLP-1 (7-36) NH 2 (60 pM) or 125 I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled peptide agonists were added for overnight incubation at 4 C

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