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The crude material was purified by automated flash column chromatography on silica via gradient elution, from 100% dichloromethane to 1% methanol in dichloromethane to produce SS(NHS-CM) 2 (979 mg, 79%) as an off-white foamy solid
Serum samples were diluted three times with normal saline before analysis
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Greater decreases in postprandial glucose levels were observed with exenatide than with liraglutide or exenatide LAR [17,76]
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Consistent with previous studies, cotadutide outperformed GLP-1 alone (40 nmol/kg/day liraglutide) in achieving maximal weight loss in obese mice, and this was attributed to the increased energy expenditure (77)
