Finally, the sections were counterstained with hematoxylin and then examined under a Zeiss microscope in bright field mode
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The non-12a-OH BAs in the tissues of mice were calculated by summing the values of BA species, including MCA, MCA, UDCA, HDCA, CDCA, LCA, T-MCA, T-MCA, TUDCA, THDCA, TCDCA, TLCA, GUDCA, GCDCA, GLCA