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Description

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Expression levels of phosphor-Erk (p-Erk), p-JNK, proliferating cell nuclear antigen (Pcna), and cyclin D1 are analyzed using Western blotting, and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) is used as the loading control

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However, regeneration efficiency remains highly genotype- and species-dependent, particularly in woody and recalcitrant horticultural crops, where tissue culture responsiveness often represents a major limitation for successful genome editing

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