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Special considerations: Some GLP-1 drugs have specific timing needs

Measurements of energy expenditure and locomotor activity HFD-induced obese C57BL/6J male mice were placed in individual small acryl calorimeter chambers with free access to HFD and water, and respiratory gas (O 2 and CO 2 ) were measured every 5 min for each individual mouse by indirect calorimetry system connected to mass spectrometer (Arco2000, ArcoSystem, Chiba, Japan)

You get clean data without spurious signals from related proteins

Obesity is linked to cardiometabolic health conditions, such as type 2 diabetes (T2D), hypertension, and hyperlipidemia [2]

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& Voet, J