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Y., Gauffre, F., Phi, T

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Method details Generation of transgenic Cryptosporidium parasites All transgenic parasites were created with IOWA strain IIA (Bunch Grass Farms, ID, USA) as the parental line using methods described previously 58,59 .Briefly, 2.5 x 10 7 oocysts were induced to excyst following 1% sodium hypochlorite treatment and incubation in 0.75% sodium taurocholate in RPMI for 45 minutes at 37C to release sporozoites

oral, IM, and IV have all appeared in published research