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For the tumour allograft model, Gclc f/f Rosa26 creERT2 MMTV-PYMT mice were euthanized and autochthonous tumours were collected, cut into 8 mm 3 fragments and cryopreserved in liquid nitrogen
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The key is gradual dose escalation, patience while steady-state levels build, and knowing that the full effect takes months to arrive
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