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( d ) GSEA analysis of data from ( b, c ) run against metabolic gene sets (defined in 10 )

After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)

But the caloric content is only part of the problem

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Liver damage or hepatitis

Insert the needle at a slight angle through the stopper