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Animals were acclimatized for one week before experimentation and maintained under controlled housing conditions (temperature 25 1 C and relative humidity 60 5%) with a 12 h light/dark cycle

Vitamin B12 is generally very safe even at high doses because it is water-soluble and excess amounts are excreted in urine

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[4] Patients are counseled to cleanse with a mild, non-foaming wash, pat dry, wait ten minutes, then apply a pea-sized amount over the entire face at bedtime

10.1016/j.ijsu.2019.07.023 16 KataokaH.YagiT.IkedoT.ImaiH.KawamuraK.YoshidaK.et al (2020)

It is administered intravenously so that it gets delivered directly into the bloodstream for immediate absorption