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Consequently, the results only considered VOCs measured in culture samples after subtracting the corresponding blank values
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5% w/v SDS, 8 M urea, 100 mM dithiothreitol, 0.02% w/v bromophenol blue) at 37 C for 10 minutes before SDS-PAGE and western blot analysis
Its considered safe by healthcare practitioners because its not absorbed into the bloodstream and has fewer side effects
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