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Cells were then fixed with 4% cold (4 C) paraformaldehyde in cytoskeleton buffer for 10 min at 4 C, permeabilized in 0.5% Triton X-100 in warm (37 C) cytoskeleton buffer at room temperature, washed twice with PBS at room temperature, and immunostained

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In the case of MCC, a more stable but comparable production was achieved, but the reaction rate was significantly lower than that of the cellulose sponge

Looking forward, several developments may help address these barriers