Glutathione and cysteine concentration measurements Choroid plexus samples were homogenized in 100 L of a 10 mM K-phosphate buffer, pH 7.4, containing 1 mM acivicin (A2295, Sigma-Aldrich) and 500 M 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), (D8130, Sigma-Aldrich) in a plastic tube, using a Teflon micro-plunger
This T4 needs to be converted into the active T3 form, triiodothyronine which is roughly four times more powerful than T4
the rate of optical density change at 412 nm is proportional to activity
Recommendations : Shake well
In UK clinical and pharmaceutical practice, Water for Injections BP (single-use, preservative-free) is the standard diluent for injectable preparations
Dozio E et al