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Semaglutide does not directly interfere with amitriptyline's antidepressant mechanisms

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However, this method is unable to discriminate between GLP-1R clustering at the plasma membrane versus bona fide endocytosis events, although these are intrinsically linked, with the former occurring rapidly after ligand stimulation as a precursor to uptake into clathrin-coated vesicles (Buenaventura et al ., 2019)

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