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Step-by-Step Instructions For How to Reconstitute Glutathione Step 1: Prepare Your Environment Work quickly and cleanly Minimize the time your solution is exposed to open air Wipe all surfaces and your hands with alcohol Keep ambient light low or work away from direct sunlight Step 2: Clean the Vial Tops Swab the tops of both the glutathione vial and the sterile water vial firmly with a fresh alcohol swab Allow both to air dry for 10 to 15 seconds before proceeding Step 3: Draw Your Reconstitution Fluid Use a fresh sterile syringe to draw the appropriate volume of sterile water for injection as specified by your compounding pharmacy or prescribing clinician Do not use saline solutions with additives that may introduce metal contamination Step 4: Inject Slowly Into the Glutathione Vial Inject the sterile water SLOWLY along the side wall of the vial (DO NOT blast it directly into the powder) Aggressive injections introduce unnecessary agitation and increase surface area contact with oxygen Step 5: Gently Swirl, Do Not Shake Gently roll and swirl the vial until the powder is fully dissolved Vigorously shaking the vial creates foam, increases oxidative surface exposure, and degrades the molecule faster Step 6: Inspect Before Use The solution should be clear to slightly pale yellow ( Discoloration is a visible signal of oxidation) Discard immediately if the solution appears cloudy, particulate, discolored brown or dark NEVER use degraded solution Step 7: Administer Immediately NON-NEGOTIABLE: Reconstituted glutathione must be used immediately, lor as close to immediately as possible Every minute at room temperature after reconstitution accelerates the GSH-to-GSSG conversion

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