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2024 June 12

Its plasma concentration decreases with age: approximately 200 ng/ml at age 20 versus 80 ng/ml at age 60, correlating with the decline in regenerative capacity

Membranes were blocked in PBST containing 5% skim milk for 1 hour at 25 C, incubated overnight at 4 C with primary antibodies, and then with secondary antibodies in PBST with 0.1% skim milk for 1 hour at 25 C

Cell Signaling Technology, Danvers, MA, USA), anti-KAP1 (1:500 dilution

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