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Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics
The amide group is acetamide (ethanamide)
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The retarded leaching of nanoclusters can be attributed to their large size in comparison with molecular dyes and it is a clear advantage for imaging purposes as dye leaching decreases the fluorescence brightness of NPs and increases the background signal resulting in unfavorable imaging inefficiency/artifacts 50
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Mechanistically, these changes appear to coincide with elevated NAD+ and activation of metabolic gene programs consistent with increased energy expenditure
