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Three weeks later, mice were terminally anaesthetised with 100 mg/kg pentobarbital and transcardially perfused with PBS containing 4% PFA, brains were collected, post-fixed in 4% PFA at 4 C overnight and cryoprotected in 30% sucrose for 23 days

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Significant weight loss such as that observed in retatrutide trials can have meaningful effects on the endocrine system, including changes to oestrogen levels, menstrual cycle regularity, and fertility

The peptide shows particular activity in musculoskeletal tissue models, where it modulates growth hormone receptor expression in fibroblast cultures

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