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The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed

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In contrast, SOCS3, which has been shown to be increased in DCs by tumor-derived substances, interacts with PKM2, resulting in decreased ATP generation under hypoxic conditions and a profound effect on the function of DCs (61)