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Cells were cultured in complete RPMI 1640/GlutaMAX medium (GIBCO, 61870-010) supplemented with 10% heat-inactivated human AB serum (Institut Jacques Boy, 201021334), 1% penicillinstreptomycin (GIBCO, 15140-122), 1% HEPES (GIBCO, 15630-056), 1% sodium pyruvate (GIBCO, 11360-039) and 1% polymyxin B (final concentration 10 g ml 1
DSIP concentrations in the blood exhibit a marked diurnal variation, correlating with circadian rhythms in humans
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Some claims also suggest it may improve muscle repair and recovery post-exercise
On top of that, alcohol can disrupt sleep, which could lead to further fatigue (although studies havent been conducted on this topic yet)
Diabetologia 4 , 5667 (1968)
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